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CellTracker™ Green CMFDA (5-chloromethylfluorescein diacetate) is a fluorescent dye well suited for monitoring cell movement or location. This dye is well retained, allowing for multigenerational tracking of cellular movements. And the green excitation/emission spectra are
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Image Search Results
Journal: Advanced Science
Article Title: Role of Tunneling Nanotubes in Arachidonic Acid Transfer and Macrophage Function Reprogramming in Intrahepatic Cholangiocarcinoma
doi: 10.1002/advs.202500148
Figure Lengend Snippet: scRNA‐seq analysis and in vitro experiments reveal tumor cell advantage in TNT formation. A) UMAP plot of epithelial cells and macrophages. B) Volcano plot showing differentially expressed genes between tumor and adjacent non‐tumor epithelial cells. C,D) GO and KEGG pathway enrichment analyses of upregulated genes in tumor cells. E,F) Representative images of homotypic TNTs formed between CMFDA‐labeled HIBEpic cells (E) and CMFDA‐HuCC‐T1 cells (F). White arrows indicate TNTs. G) Quantification of homotypic TNT connections per 100 cells (left) and percentage of TNT‐connected cells (right) between HIBEpic and HuCC‐T1. Data are presented as mean ± SD; n = 3. H,I) Heterotypic TNTs between CMFDA‐HIBEpic (H) or GFP‐HuCC‐T1 (I) and mCherry‐labeled macrophages. J) Quantification of heterotypic TNT connections per 100 cells (left) and percentage of TNT‐connected cells (right) between HIBEpic/HuCC‐T1 and macrophages. Data are presented as mean ± SD; n = 3.
Article Snippet:
Techniques: In Vitro, Labeling